Methods in enzymology. Vol. 470, Guide to yeast genetics : functional genomics, proteomics, and other systems analysis / edited by Jonathan Weissman, Christine Guthrie, Gerald R. Fink

Includes bibliographical references and indexes.

Section I. Functional genomics -- 1. Analysis of gene function using DNA microarrays / Andrew P. Capaldi -- 2. An introduction to microarray data analysis and visualization / Gregg B. Whitworth -- 3. Genome-wide approaches to monitor pre-mRNA splicing / Maki Inada and Jeffrey A. Pleiss -- 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / Philippe Lefrançois, Wei Zheng and Michael Snyder -- 5. Genome-wide mapping of nucleosomes in yeast / Oliver J. Rando -- 6. Genome-wide translational profiling by ribosome footprinting / Nicholas T. Ingolia -- Section II. Systematic genetic analysis -- 7. Synthetic genetic array (SGA) analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / Anastasia Baryshnikova ... [et al.] -- 8. Making temperature-sensitive mutants / Shay Ben-Aroya ... [et al.] -- 9. Quantitative genetic interaction mapping using the E-MAP approach / Sean R. Collins, Assen Roguev and Nevan J. Krogan -- 10. Exploring gene function and drug action using chemogenomic dosage assays / Elke Ericson ... [et al.] -- Section III. Proteomics -- 11. Yeast expression proteomics by high-resolution mass spectrometry / Tobias C. Walther, Jesper V. Olsen and Matthias Mann -- 12. High-quality binary interactome mapping / Matija Dreze ... [et al.] -- 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry-based proteomics / Bernd Bodenmiller and Ruedi Aebersold -- 14. A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells / Stephen W. Michnick ... [et al.] -- 15. Yeast lipid analysis and quantification by mass spectrometry / Xue Li Guan ... [et al.] -- 16. Mass spectrometry-based metabolomics of yeast / Christopher A. Crutchfield ... [et al.] -- Section IV. Systems Analysis -- 17. Imaging single mRNA molecules in yeast / Hyun Youk, Arjun Raj and Alexander van Oudenaarden -- 18. Reconstructing gene histories in Ascomycota fungi / Ilan Wapinski and Aviv Regev -- 19. Experimental evolution in yeast: a practical guide / Maitreya J. Dunham -- 20. Enhancing stress resistance and production phenotypes through transcriptome engineering / Felix H. Lam ... [et al.] -- Section V. Advances in Cytology/Biochemistry -- 21. Visualizing yeast chromosomes and nuclear architecture / Peter Meister ... [et al.] -- 22. Quantitative localization of chromosomal loci by immunofluorescence / Donna Garvey Brickner, William Light and Jason H. Brickner -- 23. Spinning disk confocal microscopy of yeast / Kurt Thorn -- 24. Correlative GFP-immunoelectron microscopy in yeast / Christopher Buser and Kent McDonald -- 25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / J. Ross Buchan, Tracy Nissan and Roy Parker -- 26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / Daniel Zenklusen and Robert H Singer -- 27. The Use of in vitro assays to measure endoplasmic reticulum-associated degradation (ERAD) / Jeffrey L. Brodsky -- 28. A protein transformation protocol for introducing yeast prion particles into yeast / Motomasa Tanaka -- 29. Overexpression and purification of integral membrane proteins in yeast / Franklin A. Hays, Zygy Roe-Zurz, and Robert M. Stroud -- 30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / Simon Alberti, Randal Halfmann and Susan Lindquist -- Section VI. Other fungi -- 31. Genetics and molecular biology in Candida albicans / Aaron D. Hernday ... [et al.] -- 32. Molecular genetics of Schizosaccharomyces pombe / Sarah A. Sabatinos and Susan L. Forsburg -- 33. Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans / Cheryl D. Chun and Hiten D. Madhani -- 34. The Fungal Genome Initiative and lessons learned from gen