Protein purification : principles, high resolution methods, and applications / edited by Jan-Christer Janson

Includes bibliographical references and index.

Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.